- Equine Testing
- Clinical Pathology
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- Equine probiotics
General screening for subclinical and some clinical entities in elite equine athletes is often undertaken using a battery or package of tests that are chosen by the laboratory or by the individual clinician. Low-grade abnormalities or significant clinical entities can then be further investigated, by the study of specific organ systems e.g. liver, kidney, GIT, etc.
This approach has much to recommend, but it is important to remember that the statistical probability of having one or more values outside a reference range increases can increase with the number of tests carried out, regardless of the presence of disease or other compromise. Reference ranges for frequently used biochemical tests such as total protein and globulin can be based on the selection of horses with normal WBC and plasma fibrinogen values.
Various haematology and biochemistry panels are available. Please consult our price list for further details.
1. Sample collection: Blood samples must be collected into the appropriate containers. Collection into evacuated containers has become the norm. Potassium EDTA is the anticoagulant for haematology. Lithium heparin is not suitable for this purpose, because it does not permit differential white cell counting. Samples for blood biochemistry testing can be collected into heparinised or plain tubes for either plasma or serum biochemistry respectively. Coagulation studies and the modified Clauss method of measurement of the acute phase protein fibrinogen can only be carried out using sodium citrate containers.
2. Sample technique: Samples should be collected in an aseptic manner, so as to avoid inducing sepsis, local general, into an otherwise healthy horse. The collection should be sympathetic, otherwise, you run the risk of creating the potentially misleading artefacts that result from adrenal-induced neutrophilia and splenic contraction.
3. Timing of sampling: The timing of elective sampling should reflect the questions that are being asked. There is little point in taking post-exercise samples or sampling within two or three hours of exercise if the issue is whether muscle enzymes are within the normal resting range. Samples for elective testing are best collected pre-exercise, early in the morning, or alternatively, late in the afternoon when exercise has been completed in the morning.
4. Sample handling: Misleading artefacts can also arise from poor sampling handling in the period between collection and evaluation in the laboratory. The use of an insulated lightproof container, a laboratory blood sample rack, and in hot climates, an ordinary household cold pack, can yield rich dividends in terms of the accuracy and value of the information generated from the sampling. Labeling should be legible and thought should be given to the timing of transfer for analysis.